One important point – if you have problems getting glade to run on Windows, check if you have python27.dll installed in either of
C:windowssystem32
C:windowssysWOW64
If you're running a 64 bit version of Windows, the one in system32 should be the 64 bit one (this may not seem intuitive but then this is is Microsoft…) and the one in sysWOW64 should be the 32 bit dll.
Better still just use the ones in the default installations and start Glade from that directory.
If you're not sure, try using Dependency Walker (http://www.dependencywalker.com/) which will show you just which DLL's your executable is using and whether there are any conflicts.